SP Tissue and Tumor DNA Isolation Kit
Bionano Prep SP Tissue and Tumor kit replaces our previous plug lysis Animal Tissue kit and can provide ultra-high molecular weight (UHMW) gDNA from animal tissue samples in as little as 6 hours. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation. Sample sources include human lung tumor, human liver tumor, human breast tumor, rat kidney, rat ovaries. This kit can be used for processing fresh or frozen samples.
With this protocol, up to 6 tissue samples can be processed in a day* with typical yields from 10 mg of tissue to be 6 ug and the isolated DNA can be used for imaging of cancer genomes.
|20402||4 mm nanobind disk||10 disks||room temp|
|20380||Protein LoBind Microcentrifuge Tubes, 1.5 ml||10 tubes||room temp|
|20396||Microcentrifuge Tubes, 2 ml||10 tubes||room temp|
|20403||40 μm Cell Strainer||10 each||room temp|
|20405||Detergent||150 μl||room temp|
|20404||Salting Buffer||1.1 ml||room temp|
|20406||Homogenization Buffer||96 ml||room temp|
|20407||Wash Buffer A||12 ml||room temp|
|20372||Proteinase K||0.5 ml||room temp|
|20375||Lysis and Binding Buffer (LBB)||2.5 ml||room temp|
|20376||Wash Buffer 1 Concentrate (2.5X) (WB1)||2 x 3.25 ml||room temp|
|20377||Wash Buffer 2 Concentrate (2.5X) (WB2)||5 ml||room temp|
|20378||Elution Buffer (EB)||1.1 ml||room temp|
|20381||SP Sheaths||10 each||room temp|
|SP Tissue and Tumor Kit (80038)|