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SP Tissue and Tumor DNA Isolation Kit
Bionano Prep SP Tissue and Tumor DNA Isolation kit (#80038) utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk, which binds and releases gDNA with minimal fragmentation, resulting in ultra-high molecular weight (UHMW) gDNA. This kit replaces our previous plug lysis-based Animal Tissue DNA Isolation kit (#80002).
This kit can be used for processing fresh or frozen samples. Up to 8 tissue or tumor samples can be processed in a 6-hour period, with typical yields from 10 mg of tissue to be 6 ug of UHMW gDNA. We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation (see protocol for details).
| 20402 | 4 mm nanobind disk | 10 disks | room temp | |
| 20380 | Protein LoBind Microcentrifuge Tubes, 1.5 ml | 10 tubes | room temp | |
| 20396 | Microcentrifuge Tubes, 2 ml | 10 tubes | room temp | |
| 20403 | 40 μm Cell Strainer | 10 each | room temp | |
| 20405 | Detergent | 150 μl | room temp | Download |
| 20404 | Salting Buffer | 1.1 ml | room temp | Download |
| 20406 | Homogenization Buffer | 96 ml | room temp | Download |
| 20407 | Wash Buffer A | 12 ml | room temp | Download |
| 20372 | Proteinase K | 0.5 ml | room temp | Download |
| 20375 | Lysis and Binding Buffer (LBB) | 2.5 ml | room temp | Download |
| 20376 | Wash Buffer 1 Concentrate (2.5X) (WB1) | 2 x 3.25 ml | room temp | Download |
| 20377 | Wash Buffer 2 Concentrate (2.5X) (WB2) | 5 ml | room temp | Download |
| 20378 | Elution Buffer (EB) | 1.1 ml | room temp | Download |
| 20381 | SP Sheaths | 10 each | room temp | |
| SP Tissue and Tumor Kit (80038) | ||||
|---|---|---|---|---|
| Part Number | Component | Volume | Storage | MSDS |
Protocols | |
|---|---|
| 30339 - SP Tissue and Tumor DNA Isolation Protocol, Rev A | 489 KB |
Shipping Instructions | |
|---|---|
| 30186 - Tissue and Tumor Shipping Instructions, Rev C | 60 KB |
Technical Documentation | |
|---|---|
| 30400 - SP Brain Tissue DNA Isolation Tech Note, Rev A | 653 KB |
This tech note is not a fully validated protocol. It has been only used to isolate UHMW DNA from non-tumorous healthy adult rat brain tissue and non-tumorous human brain tissue for the Bionano Saphyr system. For brain tumor tissue or other tissue/tumor types, please refer to Document 30339. | |
