SP Tissue and Tumor DNA Isolation Kit

Catalog Number: 80038

Bionano Prep SP Tissue and Tumor DNA Isolation kit (#80038) utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk, which binds and releases gDNA with minimal fragmentation, resulting in ultra-high molecular weight (UHMW) gDNA. This kit replaces our previous plug lysis-based Animal Tissue DNA Isolation kit (#80002).

This kit can be used for processing fresh or frozen samples. Up to 8 tissue or tumor samples can be processed in a 6-hour period, with typical yields from 10 mg of tissue to be 6 ug of UHMW gDNA. We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation (see protocol for details).


204024 mm nanobind disk10 disksroom temp
20380Protein LoBind Microcentrifuge Tubes, 1.5 ml10 tubesroom temp
20396Microcentrifuge Tubes, 2 ml10 tubesroom temp
2040340 μm Cell Strainer10 eachroom temp
20405Detergent150 μlroom tempDownload
20404Salting Buffer1.1 mlroom tempDownload
20406Homogenization Buffer96 mlroom tempDownload
20407Wash Buffer A12 mlroom tempDownload
20372Proteinase K0.5 mlroom tempDownload
20375Lysis and Binding Buffer (LBB)2.5 mlroom tempDownload
20376Wash Buffer 1 Concentrate (2.5X) (WB1)2 x 3.25 mlroom tempDownload
20377Wash Buffer 2 Concentrate (2.5X) (WB2)5 mlroom tempDownload
20378Elution Buffer (EB)1.1 mlroom tempDownload
20381SP Sheaths10 eachroom temp
SP Tissue and Tumor Kit (80038)
Part NumberComponentVolumeStorageMSDS

Tech Notes

Bionano SP Brain Tissue DNA Isolation Tech Note - 30400, Rev A653 KB

This tech note is not a fully validated protocol. It has been only used to isolate UHMW DNA from non-tumorous healthy adult rat brain tissue and non-tumorous human brain tissue for the Bionano Saphyr system. For brain tumor tissue or other tissue/tumor types, please refer to Document 30339.

Demonstration of the homogenization and filtration steps of the Bionano SP Prep Tissue and Tumor DNA Isolation Protocol

Contact Support

Call 858.888.7663 or email us at support@bionanogenomics.com

This website stores cookies on your computer. These cookies are used to collect information about how you interact with our website and allow us to remember you. We use this information in order to improve and customize your browsing experience and for analytics and metrics about our visitors both on this website and other media. To find out more about the cookies we use, see our Privacy Policy.