SP Tissue and Tumor DNA Isolation Kit

Catalog Number: 80038

Bionano Prep SP Tissue and Tumor kit replaces our previous plug lysis Animal Tissue kit and can provide ultra-high molecular weight (UHMW) gDNA from animal tissue samples in as little as 6 hours. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation. Sample sources include human lung tumor, human liver tumor, human breast tumor, rat kidney, rat ovaries. This kit can be used for processing fresh or frozen samples.

With this protocol, up to 6 tissue samples can be processed in a day* with typical yields from 10 mg of tissue to be 6 ug and the isolated DNA can be used for imaging of cancer genomes.

SP Tissue and Tumor Kit (80038)
20402	4 mm nanobind disk	10 disks	room temp
20380	Protein LoBind Microcentrifuge Tubes, 1.5 ml	10 tubes	room temp
20396	Microcentrifuge Tubes, 2 ml	10 tubes	room temp
20403	40 μm Cell Strainer	10 each	room temp
20405	Detergent	150 μl	room temp	Download
20404	Salting Buffer	1.1 ml	room temp	Download
20406	Homogenization Buffer	96 ml	room temp	Download
20407	Wash Buffer A	12 ml	room temp	Download
20372	Proteinase K	0.5 ml	room temp	Download
20375	Lysis and Binding Buffer (LBB)	2.5 ml	room temp	Download
20376	Wash Buffer 1 Concentrate (2.5X) (WB1)	2 x 3.25 ml	room temp	Download
20377	Wash Buffer 2 Concentrate (2.5X) (WB2)	5 ml	room temp	Download
20378	Elution Buffer (EB)	1.1 ml	room temp	Download
20381	SP Sheaths	10 each	room temp

Part Number	Component	Volume	Storage	MSDS