SP Tissue and Tumor DNA Isolation Kit

Bionano Prep SP Tissue and Tumor kit replaces our previous plug lysis Animal Tissue kit and can provide ultra-high molecular weight (UHMW) gDNA from animal tissue samples in as little as 6 hours. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation. Sample sources include human lung tumor, human liver tumor, human breast tumor, rat kidney, rat ovaries. This kit can be used for processing fresh or frozen samples.

With this protocol, up to 6 tissue samples can be processed in a day* with typical yields from 10 mg of tissue to be 6 ug and the isolated DNA can be used for imaging of cancer genomes.

204024 mm nanobind disk10 disksroom temp
20380Protein LoBind Microcentrifuge Tubes, 1.5 ml10 tubesroom temp
20396Microcentrifuge Tubes, 2 ml10 tubesroom temp
2040340 μm Cell Strainer10 eachroom temp
20405Detergent150 μlroom temp
20404Salting Buffer1.1 mlroom temp
20406Homogenization Buffer96 mlroom temp
20407Wash Buffer A12 mlroom temp
20372Proteinase K0.5 mlroom temp
20375Lysis and Binding Buffer (LBB)2.5 mlroom temp
20376Wash Buffer 1 Concentrate (2.5X) (WB1)2 x 3.25 mlroom temp
20377Wash Buffer 2 Concentrate (2.5X) (WB2)5 mlroom temp
20378Elution Buffer (EB)1.1 mlroom temp
20381SP Sheaths10 eachroom temp
SP Tissue and Tumor Kit (80038)
Part NumberComponentVolumeStorage
Ordering
Information
Catalog Number:
80038
Number of Preps: 10

FAQs

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Videos

Bionano SP Prep Tissue and Tumor DNA Isolation Protocol Video Tutorial - Homogenization and Filtration
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