Bionano Prep SP Tissue and Tumor DNA Isolation kit (#80038) utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk, which binds and releases gDNA with minimal fragmentation, resulting in ultra-high molecular weight (UHMW) gDNA. This kit replaces our previous plug lysis-based Animal Tissue DNA Isolation kit (#80002).
This kit can be used for processing fresh or frozen samples. Up to 8 tissue or tumor samples can be processed in a 6-hour period, with typical yields from 10 mg of tissue to be 6 ug of UHMW gDNA. We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation (see protocol for details).
This tech note is not a fully validated protocol. It has been only used to isolate UHMW DNA from non-tumorous healthy adult rat brain tissue and non-tumorous human brain tissue for the Bionano Saphyr system. For brain tumor tissue or other tissue/tumor types, please refer to Document 30339.