SP Tissue and Tumor DNA Isolation Kit
Bionano Prep SP Tissue and Tumor DNA Isolation kit (#80038) utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk, which binds and releases gDNA with minimal fragmentation, resulting in ultra-high molecular weight (UHMW) gDNA. This kit replaces our previous plug lysis-based Animal Tissue DNA Isolation kit (#80002).
This kit can be used for processing fresh or frozen samples. Up to 8 tissue or tumor samples can be processed in a 6-hour period, with typical yields from 10 mg of tissue to be 6 ug of UHMW gDNA. We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation (see protocol for details).
|20402||4 mm nanobind disk||10 disks||room temp|
|20380||Protein LoBind Microcentrifuge Tubes, 1.5 ml||10 tubes||room temp|
|20396||Microcentrifuge Tubes, 2 ml||10 tubes||room temp|
|20403||40 μm Cell Strainer||10 each||room temp|
|20405||Detergent||150 μl||room temp||Download|
|20404||Salting Buffer||1.1 ml||room temp||Download|
|20406||Homogenization Buffer||96 ml||room temp||Download|
|20407||Wash Buffer A||12 ml||room temp||Download|
|20372||Proteinase K||0.5 ml||room temp||Download|
|20375||Lysis and Binding Buffer (LBB)||2.5 ml||room temp||Download|
|20376||Wash Buffer 1 Concentrate (2.5X) (WB1)||2 x 3.25 ml||room temp||Download|
|20377||Wash Buffer 2 Concentrate (2.5X) (WB2)||5 ml||room temp||Download|
|20378||Elution Buffer (EB)||1.1 ml||room temp||Download|
|20381||SP Sheaths||10 each||room temp|
|SP Tissue and Tumor Kit (80038)|
|30339 - SP Tissue and Tumor DNA Isolation Protocol, Rev A||489 KB|
|30186 - Tissue and Tumor Shipping Instructions, Rev C||60 KB|
|30400 - SP Brain Tissue DNA Isolation Tech Note, Rev A||653 KB|
This tech note is not a fully validated protocol. It has been only used to isolate UHMW DNA from non-tumorous healthy adult rat brain tissue and non-tumorous human brain tissue for the Bionano Saphyr system. For brain tumor tissue or other tissue/tumor types, please refer to Document 30339.