SP Tissue and Tumor DNA Isolation Kit

Catalog Number: 80038

Bionano Prep SP Tissue and Tumor kit replaces our previous plug lysis Animal Tissue kit and can provide ultra-high molecular weight (UHMW) gDNA from animal tissue samples in as little as 6 hours. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

We have validated a wide variety of tissue types and sources with this kit for successful UHMW DNA isolation. Sample sources include human lung tumor, human liver tumor, human breast tumor, rat kidney, rat ovaries. This kit can be used for processing fresh or frozen samples.

With this protocol, up to 6 tissue samples can be processed in a day* with typical yields from 10 mg of tissue to be 6 ug and the isolated DNA can be used for imaging of cancer genomes.

204024 mm nanobind disk10 disksroom temp
20380Protein LoBind Microcentrifuge Tubes, 1.5 ml10 tubesroom temp
20396Microcentrifuge Tubes, 2 ml10 tubesroom temp
2040340 μm Cell Strainer10 eachroom temp
20405Detergent150 μlroom tempDownload
20404Salting Buffer1.1 mlroom tempDownload
20406Homogenization Buffer96 mlroom tempDownload
20407Wash Buffer A12 mlroom tempDownload
20372Proteinase K0.5 mlroom tempDownload
20375Lysis and Binding Buffer (LBB)2.5 mlroom tempDownload
20376Wash Buffer 1 Concentrate (2.5X) (WB1)2 x 3.25 mlroom tempDownload
20377Wash Buffer 2 Concentrate (2.5X) (WB2)5 mlroom tempDownload
20378Elution Buffer (EB)1.1 mlroom tempDownload
20381SP Sheaths10 eachroom temp
SP Tissue and Tumor Kit (80038)
Part NumberComponentVolumeStorageMSDS
Bionano SP Prep Tissue and Tumor DNA Isolation Protocol Video Tutorial - Homogenization and Filtration

Contact Support

Call 858.888.7663 or email us at support@bionanogenomics.com

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