SP Bone Marrow Aspirate DNA Isolation Kit

Catalog Number: 90103

Bionano Prep SP BMA DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA from white blood cells in heparin BMA samples in as little as 4 hours. BMA samples are pre-processed by filtering through a 100 μm filter (supplied with the kit) to remove any bone fragments and insoluble/non-cellular particulates. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

With this protocol, up to eight BMA samples can be processed in a day* with typical yields from 1.5 million cells as high as 7 μg.  While fresh BMA samples can be processed with this kit, we have observed higher molecule N50s (> 400 kbp) when samples are frozen with DNA stabilizer and processed after a single freeze-thaw cycle.

*Processing time may vary depending upon the number of times filtered samples need to be diluted in order to be counted accurately. Once 1.5 million cells are obtained, the total processing time is less than 4 hours.

Note: The protocol listed below is for use with the updated v2 version of the kit (PN 90103). For access to the protocol for use with the previous version (PN 90057), please contact support@bionanogenomics.com or your local Bionano representative.

20373RNase A Enzyme200 μl4°CDownload
20423DNA Stabilizer350 μlRoom tempDownload
20374Cell Buffer50 mlRoom tempDownload
20372Proteinase K Enzyme0.5 mlRoom tempDownload
20375Lysis and Binding Buffer2.5 mlRoom tempDownload
20376Wash Buffer 1 Concentrate3.25 mlRoom tempDownload
20377Wash Buffer 2 Concentrate5 mlRoom tempDownload
20378Elution Buffer1.1 mlRoom tempDownload
204024 mm Nanobind Disks10 disksRoom temp
20422Protein LoBind Tubes, 1.5 mL10 tubesRoom temp
20421Protein LoBind Tubes, 0.5 mL10 tubesRoom temp
20396Standard Microfuge Tubes10 tubesRoom temp
20381Disk Retreiver Sheath10 eachRoom temp
20398DNA Stabilizer4 mlRoom temp
20396Standard Microfuge2 x 10 tubesRoom temp
20401BMA Filters, 100 μm20 filtersRoom temp
Kit Contents (SP BMA)
Part NumberComponentVolumeStorageMSDS
Demonstration of the HemoCue WBC Analyzer for Bionano Prep SP DNA Isolation

Contact Support

Call 858.888.7663 or email us at support@bionanogenomics.com

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