Bionano Prep SP BMA DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA from white blood cells in heparin BMA samples in as little as 4 hours. BMA samples are pre-processed by filtering through a 100 μm filter (supplied with the kit) to remove any bone fragments and insoluble/non-cellular particulates. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
With this protocol, up to eight BMA samples can be processed in a day* with typical yields from 1.5 million cells as high as 7 μg. While fresh BMA samples can be processed with this kit, we have observed higher molecule N50s (> 400 kbp) when samples are frozen with DNA stabilizer and processed after a single freeze-thaw cycle.
*Processing time may vary depending upon the number of times filtered samples need to be diluted in order to be counted accurately. Once 1.5 million cells are obtained, the total processing time is less than 4 hours.
Note: The protocol listed below is for use with the updated v2 version of the kit (PN 90103). For access to the protocol for use with the previous version (PN 90057), please contact email@example.com or your local Bionano representative.