SP Bone Marrow Aspirate DNA Isolation Kit
Bionano Prep SP BMA DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA from white blood cells in heparin BMA samples in as little as 4 hours. BMA samples are pre-processed by filtering through a 100 μm filter (supplied with the kit) to remove any bone fragments and insoluble/non-cellular particulates. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
With this protocol, up to eight BMA samples can be processed in a day* with typical yields from 1.5 million cells as high as 7 μg. While fresh BMA samples can be processed with this kit, we have observed higher molecule N50s (> 400 kbp) when samples are frozen with DNA stabilizer and processed after a single freeze-thaw cycle.
*Processing time may vary depending upon the number of times filtered samples need to be diluted in order to be counted accurately. Once 1.5 million cells are obtained, the total processing time is less than 4 hours.
Note: The protocol listed below is for use with the updated v2 version of the kit (PN 90103). For access to the protocol for use with the previous version (PN 90057), please contact email@example.com or your local Bionano representative.
|20373||RNase A Enzyme||200 μl||4°C||Download|
|20397||DNA Stabilizer||20 μl||Room temp||Download|
|20374||Cell Buffer||50 ml||Room temp||Download|
|20372||Proteinase K Enzyme||0.5 ml||Room temp||Download|
|20375||Lysis and Binding Buffer||2.5 ml||Room temp||Download|
|20376||Wash Buffer 1 Concentrate||3.25 ml||Room temp||Download|
|20377||Wash Buffer 2 Concentrate||5 ml||Room temp||Download|
|20378||Elution Buffer||1.1 ml||Room temp||Download|
|20402||4 mm Nanobind Disks||10 disks||Room temp|
|20422||Protein LoBind Tubes, 1.5 mL||10 tubes||Room temp|
|20421||Protein LoBind Tubes, 0.5 mL||10 tubes||Room temp|
|20396||Standard Microfuge Tubes||10 tubes||Room temp|
|20381||Disk Retreiver Sheath||10 each||Room temp|
|20398||DNA Stabilizer||4 ml||Room temp|
|20396||Standard Microfuge||2 x 10 tubes||Room temp|
|20401||BMA Filters, 100 μm||20 filters||Room temp|
|Kit Contents (SP BMA)|
|Bionano Prep SP BMA DNA Isolation Protocol v2 - 30399, Rev A||541 KB|
|Bone Marrow Aspirate Shipping Instructions - 30358, Rev A||63 KB|
|BMA Kit Flyer - 30327, Rev B||290 KB|