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SP Bone Marrow Aspirate DNA Isolation Kit
Bionano Prep SP BMA DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA from white blood cells in heparin BMA samples in as little as 4 hours. BMA samples are pre-processed by filtering through a 100 μm filter (supplied with the kit) to remove any bone fragments and insoluble/non-cellular particulates. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
With this protocol, up to eight BMA samples can be processed in a day* with typical yields from 1.5 million cells as high as 7 μg. While fresh BMA samples can be processed with this kit, we have observed higher molecule N50s (> 400 kbp) when samples are frozen with DNA stabilizer and processed after a single freeze-thaw cycle.
| 20373 | RNase A Enzyme | 200 μl | 4°C | Download |
| 20397 | DNA Stabilizer | 20 μl | Room temp | Download |
| 20374 | Cell Buffer | 50 ml | Room temp | Download |
| 20372 | Proteinase K Enzyme | 0.5 ml | Room temp | Download |
| 20375 | Lysis and Binding Buffer | 2.5 ml | Room temp | Download |
| 20376 | Wash Buffer 1 Concentrate | 3.25 ml | Room temp | Download |
| 20377 | Wash Buffer 2 Concentrate | 5 ml | Room temp | Download |
| 20378 | Elution Buffer | 1.1 ml | Room temp | Download |
| 20379 | Nanobind Disks | 10 disks | Room temp | |
| 20380 | Protein LoBind Tubes | 20 tubes | Room temp | |
| 20396 | Standard Microfuge Tubes | 10 tubes | Room temp | |
| 20381 | Disk Retreiver Sheath | 10 each | Room temp | |
| 20398 | DNA Stabilizer | 4 ml | Room temp | |
| 20396 | Standard Microfuge | 2 x 10 tubes | Room temp | |
| 20401 | BMA Filters, 100 μm | 20 filters | Room temp | |
| Kit Contents (SP BMA) | ||||
|---|---|---|---|---|
| Part Number | Component | Volume | Storage | MSDS |
Information
