SP Blood & Cell Culture DNA Isolation Kit

Catalog Number: 80042

This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures. It utilizes a lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

Note: The protocols listed below are for use with the updated v2 version of the kit (PN 80042). For access to the protocols for use with the previous version (PN 80030), please contact support@bionanogenomics.com or your local Bionano representative.

20373RNase A Enzyme200 μl4°CDownload
20423DNA Stabilizer350 μlRoom tempDownload
20374Cell Buffer50 mlRoom tempDownload
20372Proteinase K Enzyme0.5 mlRoom tempDownload
20375Lysis and Binding Buffer2.5 mlRoom tempDownload
20376Wash Buffer 1 Concentrate3.25 mlRoom tempDownload
20377Wash Buffer 2 Concentrate5 mlRoom tempDownload
20378Elution Buffer1.1 mlRoom tempDownload
204024 mm Nanobind Disks10 disksRoom temp
20422Protein LoBind Tubes, 1.5 mL10 tubesRoom temp
20421Protein LoBind Tubes, 0.5 mL10 tubesRoom temp
20396Standard Microfuge Tubes10 tubesRoom temp
20381Disk Retreiver Sheath10 eachRoom temp
Part NumberComponentVolumeStorageMSDS

Tech Notes

Bionano Prep SP Frozen Cryopreserved Cell DNA Isolation Technote - TECHN-00001, Rev 01841 KB

This Bionano Prep SP Cryopreserved Cell Tech Note is not a fully validated protocol and has only been used to isolate ultra-high molecular weight (UHMW) DNA from cryopreserved lymphoblastoid cell lines, peripheral blood mononuclear cells (PBMC), and bone marrow mononuclear cells (BMNC) for the Bionano Saphyr system. For samples stored as frozen cell pellets, please refer to document 30398, Bionano Prep SP Frozen Cell Pellet DNA Isolation Protocol v2.

Demonstration of the bind, wash, and elute steps of the Bionano Prep SP Frozen Blood DNA Isolation Protocol
Demonstration of the HemoCue WBC Analyzer for Bionano Prep SP DNA Isolation

Contact Support

Call 858.888.7663 or email us at support@bionanogenomics.com

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