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SP Blood & Cell Culture DNA Isolation Kit
This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures. It utilizes a lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
First-time users must order Bionano Prep SP Magnetic Retriever (#80031).
Learn how to isolate DNA with higher N50s from WBCs with a minor modification
| 20373 | RNase A Enzyme | 200 μl | 4°C | Download |
| 20397 | DNA Stabilizer | 20 μl | Room temp | Download |
| 20374 | Cell Buffer | 50 ml | Room temp | Download |
| 20372 | Proteinase K Enzyme | 0.5 ml | Room temp | Download |
| 20375 | Lysis and Binding Buffer | 2.5 ml | Room temp | Download |
| 20376 | Wash Buffer 1 Concentrate | 3.25 ml | Room temp | Download |
| 20377 | Wash Buffer 2 Concentrate | 5 ml | Room temp | Download |
| 20378 | Elution Buffer | 1.1 ml | Room temp | Download |
| 20379 | Nanobind Disks | 10 disks | Room temp | |
| 20380 | Protein LoBind Tubes | 20 tubes | Room temp | |
| 20396 | Standard Microfuge Tubes | 10 tubes | Room temp | |
| 20381 | Disk Retreiver Sheath | 10 each | Room temp | |
| KIT CONTENTS | ||||
|---|---|---|---|---|
| Part Number | Component | Volume | Storage | MSDS |
Ordering
Information
Information
Catalog Number:
80030
Number of Preps:
10
Contact Information:
+1-858-888-7600
Downloads
Protocols and Guides
- Bionano Prep SP Fresh Human Blood DNA Isolation Protocol - 30258, Rev D
516 KB
Download - Bionano Prep SP Frozen Human Blood DNA Isolation Protocol - 30246, Rev E
518 KB
Download - Bionano Prep SP Fresh Cells DNA Isolation Protocol - 30257, Rev D
509 KB
Download - Bionano Prep SP Frozen Cell Pellet DNA Isolation Protocol - 30268, Rev D
509 KB
Download - Cell Line Shipping Instructions - 30180, Rev C
64 KB
Download - Blood Shipping Instructions - 30179, Rev C
61 KB
Download
FAQs
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