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SP Blood & Cell Culture DNA Isolation Kit
This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures. It utilizes a lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.
Note: The protocols listed below are for use with the updated v2 version of the kit (PN 80042). For access to the protocols for use with the previous version (PN 80030), please contact support@bionanogenomics.com or your local Bionano representative.
| 20373 | RNase A Enzyme | 200 μl | 4°C | Download |
| 20423 | DNA Stabilizer | 350 μl | Room temp | Download |
| 20374 | Cell Buffer | 50 ml | Room temp | Download |
| 20372 | Proteinase K Enzyme | 0.5 ml | Room temp | Download |
| 20375 | Lysis and Binding Buffer | 2.5 ml | Room temp | Download |
| 20376 | Wash Buffer 1 Concentrate | 3.25 ml | Room temp | Download |
| 20377 | Wash Buffer 2 Concentrate | 5 ml | Room temp | Download |
| 20378 | Elution Buffer | 1.1 ml | Room temp | Download |
| 20402 | 4 mm Nanobind Disks | 10 disks | Room temp | |
| 20422 | Protein LoBind Tubes, 1.5 mL | 10 tubes | Room temp | |
| 20421 | Protein LoBind Tubes, 0.5 mL | 10 tubes | Room temp | |
| 20396 | Standard Microfuge Tubes | 10 tubes | Room temp | |
| 20381 | Disk Retreiver Sheath | 10 each | Room temp | |
| KIT CONTENTS | ||||
|---|---|---|---|---|
| Part Number | Component | Volume | Storage | MSDS |
Tech Notes | |
|---|---|
| Bionano Prep SP Frozen Cryopreserved Cell DNA Isolation Technote - TECHN-00001, Rev 01 | 841 KB |
This Bionano Prep SP Cryopreserved Cell Tech Note is not a fully validated protocol and has only been used to isolate ultra-high molecular weight (UHMW) DNA from cryopreserved lymphoblastoid cell lines, peripheral blood mononuclear cells (PBMC), and bone marrow mononuclear cells (BMNC) for the Bionano Saphyr system. For samples stored as frozen cell pellets, please refer to document 30398, Bionano Prep SP Frozen Cell Pellet DNA Isolation Protocol v2. | |
Troubleshooting Guide | |
|---|---|
| Bionano Prep SP and DLS Kit Troubleshooting Guide - 30488, Rev A | 174 KB |
Shipping Instructions | |
|---|---|
| Cell Line Shipping Instructions - 30180, Rev C | 72 KB |
| Blood Shipping Instructions - 30179, Rev D | 61 KB |
