SP Blood & Cell Culture DNA Isolation Kit

Catalog Number: 80042

This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures. It utilizes a lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

A new Bionano Prep® SP Amnio and CVS Culture DNA Isolation Protocol is now available to a defined set of customers. To apply for consideration in testing the new protocol, please fill out the information request form.

Note: The protocols listed below are for use with the updated v2 version of the kit (PN 80042). For access to the protocols for use with the previous version (PN 80030), please contact support@bionanogenomics.com or your local Bionano representative.

20373RNase A Enzyme200 μl4°CDownload
20423DNA Stabilizer350 μlRoom tempDownload
20374Cell Buffer50 mlRoom tempDownload
20372Proteinase K Enzyme0.5 mlRoom tempDownload
20375Lysis and Binding Buffer2.5 mlRoom tempDownload
20376Wash Buffer 1 Concentrate3.25 mlRoom tempDownload
20377Wash Buffer 2 Concentrate5 mlRoom tempDownload
20378Elution Buffer1.1 mlRoom tempDownload
204024 mm Nanobind Disks10 disksRoom temp
20422Protein LoBind Tubes, 1.5 mL10 tubesRoom temp
20421Protein LoBind Tubes, 0.5 mL10 tubesRoom temp
20396Standard Microfuge Tubes10 tubesRoom temp
20381Disk Retreiver Sheath10 eachRoom temp
KIT CONTENTS
Part NumberComponentVolumeStorageMSDS
Demonstration of HemoCue WBC Counter for Bionano DNA Isolation

Contact Support

Call 858.888.7663 or email us at support@bionanogenomics.com
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