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Nick Label Repair and Stain (NLRS)
1st generation, 5 hour labeling process for 10 samples, using site-specific nickases.
Bionano Prep™ DNA Labeling Kit reagents are used specifically for applications on Bionano systems. Fluorescent nucleotides are incorporated into specific sequence motifs on high-molecular-weight DNA, purified using the appropriate Bionano DNA Isolation Kit. The protocol specifically Nicks, Labels, Repairs and Stains (NLRS) the double stranded genomic DNA at sequence-specific sites, resulting in a uniquely identifiable genome-specific label patterns that enable de novo map assembly, anchoring of sequence contigs and discovery of large structural variation (> 1000 bp with NLRS).
20161 | 4x Flow Buffer | 188 uL | 4°C | Download |
20164 | Stop Solution | 13 uL | 4°C | Download |
20165 | Ultra Pure Water | 220 uL | 4°C | Download |
20168 | 10x Labeling Buffer | 19 uL | 4°C | Download |
20166 | 5x DTT | 150 uL | -20°C | Download |
20167 | 10x Labeling Mix | 19 uL | -20°C | Download |
20250 | 50x Repair Mix | 5 uL | -20°C | Download |
20251 | 10x Buffer 3 | 13 uL | -20°C | Download |
20258 | 10x Buffer 2 | 13 uL | -20°C | Download |
20162 | DNA Stain | 75 uL | -20°C | Download |
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Part Number | Component | Volume | Storage | MSDS |