Nick Label Repair and Stain (NLRS)

1st generation, 5 hour labeling process for 10 samples, using site-specific nickases.

Bionano Prep™ DNA Labeling Kit reagents are used specifically for applications on Bionano systems. Fluorescent nucleotides are incorporated into specific sequence motifs on high-molecular-weight DNA, purified using the appropriate Bionano DNA Isolation Kit. The protocol specifically Nicks, Labels, Repairs and Stains (NLRS) the double stranded genomic DNA at sequence-specific sites, resulting in a uniquely identifiable genome-specific label patterns that enable de novo map assembly, anchoring of sequence contigs and discovery of large structural variation (> 1000 bp with NLRS).