Direct Label and Stain (DLS)

Catalog Number: 80005

Bionano Prep DLS Labeling Kit reagents are used specifically for applications on Saphyr systems. A specific sequence motif is labeled directly with fluorophores in a single enzymatic reaction on high-molecular-weight DNA purified using the appropriate Bionano Prep DNA Isolation Kit. The Direct Label and Stain (DLS) process results in uniquely identifiable, genome-specific label patterns on double-stranded genomic DNA that enable de novo assembly, anchoring of sequence contigs, and discovery of structural variants (> 500 bp).

2035120x DLE-118 ul-20°CDownload
2035220x DL-Green18 ul-20°CDownload
203541M DTT75 ul-20°CDownload
20356DNA Stain65 ul-20°CDownload
203505x DLE-1 Buffer200 ul-20°CDownload
203534x Flow Buffer190 ul4°CDownload
20355Ultrapure Water (Nuclease Free)900 ulRoom tempDownload
20357DLS 24 Well Plate1 plateRoom temp
20358DLS Membranes (13 mm)25Room temp
20361DLS Plate Sealing Strips10Room temp
20362DLS Amber Tubes, Round Bottom12Room temp
Part NumberComponentVolumeStorageMSDS
Demonstration of proper technique for mixing the DLE-1 Master Mix
Demonstration of the Membrane Adsorption steps of the DLS protocol

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