Direct Label and Stain (DLS)

Bionano Prep™ DNA Labeling Kit reagents are used specifically for applications on Saphyr systems. A specific sequence motif is labeled directly with fluorophores in a single enzymatic reaction on high-molecular-weight DNA purified using the appropriate Bionano DNA Isolation Kit. Direct Label and Stain (DLS) results in uniquely identifiable genome-specific label patterns on the double stranded genomic DNA that enable de novo map assembly, anchoring of sequence contigs, and discovery of structural variation (> 500 bp).