The de novo assembled Bionano optical maps can be integrated with a sequence assembly to order and orient sequence fragments accurately, identify and correct potential chimeric joins in the sequence assembly, and estimate the gap size between adjacent contigs. Sequence contigs are aligned to de novo Bionano optical maps using BIonano Solve Software. Hybrid scaffolds are generated by resolving conflicts between sequence contigs and Bionano maps and can be exported as FASTA and AGP files. This pipeline is fully integrated with Bionano Access™ which provides a convenient interface for running the Hybrid Scaffold pipeline and viewing scaffolding results.
- Discovery Research /
- Genome Assembly
Improve assembly contiguity and automatically correct errors by scaffolding short and long read sequencing contigs with Bionano maps.
Generate Error-free Genome Assemblies
Most higher organisms have repetitive genomes and many repeats are inaccessible to sequencing. Long-range scaffolding is needed for a contiguous and accurate de novo assembly. Scaffolding sequence contigs with Bionano maps enables a correct assembly in chromosome arms or full chromosomes. Bionano is the only orthogonal, non-sequencing scaffolding technology, and is thus able to correct chimeric contigs and scaffolding errors caused by Hi-C. With a total output of up to 5 Tbp of data per sample, even the largest genomes can be scaffolded successfully.