• Home/
  • Restriction Site Detection in Optical Mapping Data

Restriction Site Detection in Optical Mapping Data

Advances in Intelligent Networking and Collaborative Systems 2022
Dolezi V, et al

Vít Doleží, Petr Gajdoš, Marek Běhálek, Michal Vašínek

Optical Mapping is a method of DNA sequencing that is used to detect large structural variations in genomes. To create these optical maps a restriction enzyme is mixed with DNA. The enzyme binds to DNA and creates labels called restriction sites. These labels are captured by a camera where they appear as bright spots. This work introduces two methods to find these high-intensity points in optical maps. The first method is trying to find the peaks based only on intensity levels and the second is using a signal-to-noise ratio. Both methods have more than three parameters that can affect the results. Differential evolution and particle swarm optimization were used to find the best parameters that would give the highest accuracy. Bionano results were used as ground truth.


This website stores cookies on your computer. These cookies are used to collect information about how you interact with our website and allow us to remember you. We use this information in order to improve and customize your browsing experience and for analytics and metrics about our visitors both on this website and other media. To find out more about the cookies we use, see our Privacy Policy.