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Barcode aware adaptive sampling for Oxford Nanopore sequencers.

bioRxiv 2021
Payne A, et al.

Alexander Payne, Rory Munro, Nadine Holmes, Christopher Moore, Matthew Carlile, Matthew W Loose

Adaptive sampling enables selection of individual molecules from sequencing libraries, a unique property of nanopore sequencing. Here we develop our adaptive sampling tool readfish to become “barcode-aware” enabling selection of different targets within barcoded samples or filtering out individual barcodes. We show that multiple human genomes can be assessed for copy number and structural variation on a single sequencing flow cell using sample specific customised target panels.

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