Alexander Payne, Rory Munro, Nadine Holmes, Christopher Moore, Matthew Carlile, Matthew W Loose
Adaptive sampling enables selection of individual molecules from sequencing libraries, a unique property of nanopore sequencing. Here we develop our adaptive sampling tool readfish to become “barcode-aware” enabling selection of different targets within barcoded samples or filtering out individual barcodes. We show that multiple human genomes can be assessed for copy number and structural variation on a single sequencing flow cell using sample specific customised target panels.
