Hybrid scaffold constructions
The de novo assembled Bionano optical maps can be integrated with a sequence assembly to order and orient sequence fragments accurately, identify and correct potential chimeric joins in the sequence assembly, and estimate the gap size between adjacent contigs. Sequence contigs are aligned to de novo Bionano optical maps using Bionano Solve Software. Hybrid scaffolds are generated by resolving conflicts between sequence contigs and Bionano maps and can be exported as FASTA and AGP files. This pipeline is fully integrated with Bionano Access™ which provides a convenient interface for running the Hybrid Scaffold pipeline and viewing scaffolding results.