Plant Genome Research
Progress in plant genome research is vital to understanding natural evolution and feeding the world. The size and highly repetitive nature of plant genomes make building quality reference genomes a challenge for short-read sequencing technologies. Bionano Saphyr™ offers hybrid scaffolding and structural variation discovery capabilities that are essential for driving progress.
Short-read technologies typically fail to span long repeat regions or disambiguate different copies of interspersed repeats, resulting in limited contig length, introduction of chimeric joins and other assembly errors. Unlike next-generation sequencing (NGS), which algorithmically infers structural variations from fragmented DNA data, Bionano optical genome mapping directly observes long DNA molecules by linearizing and imaging DNA in its native state using massively parallel NanoChannels.
De novo Saphyr genome maps can be integrated with sequence assembly to order and orient sequence fragments, identify and correct potential chimeric joins in the sequence assembly, and estimate the gap size between adjacent sequences.
Bionano hybrid scaffolding considerably reduces the number of contigs found in the initial NGS assembly and improves contiguity up to 100 times over NGS-based approaches alone.
The combination of Bionano maps with sequencing data improves assembly accuracy and quality while reducing the need for deep sequencing coverage. This improved contiguity significantly lowers costs associated with assembling reference genomes.
Saphyr also offers unparalleled sensitivity for large structural variations from 1,000 bp to megabase pair lengths.
- 99% sensitivity for large homozygous insertions and deletions
- 87% sensitivity for large heterozygous insertions and deletions
- 98% sensitivity for translocations
Saphyr provides this performance with a false positive rate of less than 3%. Saphyr also detects inversions, repeats, copy number variants and complex rearrangements.
See below for publications, white papers and other resources regarding Bionano genome mapping in plant genome research.
- Large-scale breeding– identify areas of biological interest for crossbreeding and massively screen previous crosses in existing plant species
- Evolutionary biology – see the complete picture of how genomes have evolved and been reorganized
- Gene discovery– call structural variations to identify variants of interest and elucidate their effect on genes within a plant species
- Reference genome assembly– perform de novo assembly and scaffold assemblies generated by sequencing-based systems
- Plant Biotechnology Journal 2017
- Hybrid assembly of the large and highly repetitive genome of Aegilops tauschii, a progenitor of bread wheat, with the mega-reads algorithmbioRxiv 2016
- The genome sequence of allopolyploid Brassica juncea and analysis of differential homoeolog gene expression influencing selectionNature Genetics 2016